pHYBi-Wiki

In this section we aim to provide a deeper understanding of key topics relevant to the pHYBi project, including topics such as plant management, polluted soils, lignocellulosic biomass and its valorisation, and more.

Phytomanagement

Phytoremediation refers to the use of plants to remove, degrade, or stabilize contaminants in the environment, such as polluted soil, water, or air. It is primarily focused on cleaning up or remediating contaminated sites. Phytoremediation can involve various processes like:

  • Phytoextraction: plant uptake of contaminants
  • Phytodegradation: breakdown of pollutants
  • Phytostabilization: immobilization of harmful substances, reducing their spread

Bioremediation is a broader term that involves using living organisms—plants, microbes, or fungi—to clean up pollutants in the environment.

Phytomanagement, on the other hand, is a broader concept that not only focuses on remediating pollutants (like in phytoremediation) but also on utilizing plants for land management and recovery purposes, such as biomass production. Phytomanagement might involve planting specific crops or vegetation to restore soil fertility, prevent erosion, or promote sustainable land use practices in addition to cleaning up contaminants. It offers a cost-effective alternative to traditional cleanup methods and provides possible applications for a bio-based economy. However, it can be time-consuming and limited by the plants’ growth rate and root depth.

Plants utilize various mechanisms to remove contaminants from soil and water during phytoremediation. These mechanisms include phytoextraction, rhizofiltration, phytostabilization, phytovolatilization, and phytodegradation:

  • Phytoextraction: Plants absorb contaminants, like heavy metals, through their roots and accumulate them in their aboveground parts (stems, leaves, etc.), making it easier to remove pollutants.
  • Rhizofiltration: Plant roots filter contaminants from water by absorbing, adsorbing, or precipitating pollutants like metals, preventing them from spreading.
  • Phytostabilization: Plants immobilize contaminants in the soil, reducing their bioavailability and preventing their spread through root accumulation.
  • Phytovolatilization: Plants take up contaminants and release them into the atmosphere in a modified, often less toxic, form.
  • Phytodegradation: Plants break down organic contaminants into simpler, less harmful compounds through natural metabolic processes.

pHYBi uses phytomanagement to tackle environmental problems such as soil contamination and degradation. The project uses a hybrid approach called nanobiophytoremediation, which combines phytoremediation and bioremediation with nanotechnology for enhanced effectiveness. This innovative method uses nanoparticles, genetically modified plants and optimised microbial consortia (including bacteria and mycorrhizae) to promote plant growth, enhance contaminant uptake and accelerate contaminant degradation. In addition, pHYBi integrates these processes with sustainable biomass production and valorisation, contributing to a circular economy by transforming harvested biomass into valuable textile applications.

pHYBi leverages a range of innovative technologies to optimize and enhance phytoremediation processes. These technologies include soil amendments, plant selection and breeding, bioaugmentation, and digital tools.

For plant selection, pHYBi uses screening and selective cultivation to identify the most suitable plant genotypes, such as poplars, for specific contaminants and environmental conditions. This helps ensure the best plants are chosen for effective phytoremediation.

In terms of bioaugmentation, pHYBi incorporates symbiotic inoculants like endophytic fungi, plant growth-promoting bacteria (PGPBs), and mycorrhizae, which enhance plant growth, health, and phytoremediation efficiency while also promoting the production of lignocellulosic biomass.

Additionally, the project employs digital tools such as genome-scale metabolic models to improve plant-microbe interactions, while drone-based remote sensing is utilized for efficient monitoring of plant and soil conditions.

To further enhance soil health and pollutant removal, pHYBi applies organic and inorganic amendments, including nanoparticles and organic fertilizers, which improve soil structure, enhance pollutant immobilization, and facilitate extraction.

Soil microorganisms interact with plant roots to make phytoremediation more effective. These interactions take place mainly in the rhizosphere, the area of soil around plant roots. Microbes can help by breaking down contaminants, making them easier for plants to absorb. They also promote plant growth and can form beneficial partnerships with plant roots, such as mycorrhizal fungi. This teamwork improves the plant’s ability to remove and detoxify contaminants from the soil.

The use of contaminated, saline soils is crucial for both environmental restoration and land efficiency. While these soils are often degraded and unproductive, methods such as phytoremediation can help restore their health, mitigate environmental risks and return them to productive use. By focusing on remediation or biomass production in these areas, disruption of undisturbed ecosystems can be avoided and a circular economy can be supported. In addition, this approach can improve soil structure and fertility, paving the way for future agricultural use and helping to combat land degradation.

Phytomanagement plays a crucial role in a circular economy by using plants to restore degraded environments, recycle nutrients, and produce valuable biomass for industrial use. This approach allows for the efficient use of land by balancing areas dedicated to food production with those utilized for biomass generation, thereby contributing to sustainable land use. The biomass produced, such as lignocellulosic materials, can be converted into bio-based products like textiles, biofuels, and bioplastics, supporting a circular bioeconomy. Phytomanagement promotes the sustainable use of natural resources, reduces waste, and aligns with the principles of sustainability, resource efficiency, and green innovation.

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Biomass valorisation

Lignocellulosic biomass refers to the main structural components of plant cell walls, primarily composed of cellulose, hemicellulose, and lignin. It is abundant, renewable, and sourced from wood, agricultural residues, energy crops, and organic waste. This biomass is an important resource for producing biofuels like ethanol and biodiesel, as well as biochemicals and bioproducts such as bioplastics and paper.

Due to its complex structure and the strong bonds between its components, processing lignocellulosic biomass requires a combination of physical, chemical, and biological treatments. Cellulose and hemicellulose can be broken down into sugars for conversion into valuable products, while lignin is utilized for energy or high-value materials.

The use of lignocellulosic biomass is crucial for advancing a circular economy as it provides a renewable and abundant resource for producing bio-based products and energy. Unlike fossil fuels, lignocellulosic biomass is derived from agricultural residues, forestry by-products, energy crops, and organic waste, making it a sustainable alternative that reduces environmental impact. By converting lignocellulosic biomass into products like biofuels, bioplastics, and biochemicals, waste materials are repurposed rather than discarded, promoting resource efficiency and minimizing waste. Additionally, its utilization supports carbon neutrality, as the carbon dioxide released during its use can be offset by the carbon captured during plant growth. Lignocellulosic biomass also enhances land-use efficiency by allowing the use of non-food crops and marginal lands for biomass production, avoiding competition with food production.

To fully realize the potential of lignocellulosic biomass in a circular economy, several challenges need to be addressed such as creating scalable, cost-efficient processing methods for diverse biomass sources to support large-scale applications.

pHYBi introduces an innovative process for lignocellulose fractionation aimed at sustainable textile production. This method combines the organosolv technique with cascade filtration to efficiently separate cellulose, lignin, and hemicellulose. Each of these components is then valorized for various textile applications:

  • Hemicellulose for pigments: The C-5 sugars from the hemicellulose fraction are used for fermentation processes to biologically produce natural pigments such as alpha-, beta-, and epsilon-carotene. These eco-friendly colorants are applied to textiles, providing sustainable dyeing solutions.
  • Cellulose and Lignin for coatings: These fractions are transformed into multifunctional protective coatings and finishes for textiles.

pHYBi ensures the safety and reliability of its produced biomass by implementing quality control measures throughout its process. The produced pigments and coatings undergo extensive testing to meet the highest industry standards, ensuring that they are safe for use and serve as sustainable alternatives to traditional products.

To assess the overall impact of the pHYBi process, the project evaluates the technical, economic, social, and environmental factors associated with the approach. This includes:

  1. Environmental analysis based on the Life Cycle Assessment (LCA) concept to evaluate the ecological impact of our production methods.

  2. Strategic economic analysis to assess the feasibility and financial sustainability of the proposed case studies.

  3. Social impact assessment using the social-LCA concept to understand the effects of our process on communities and stakeholders.

  4. Integrated evaluation of the technical, economic, environmental, and social dimensions, followed by the development of recommendations and guidelines for the effective implementation of the pHYBi concept.

Through these comprehensive analyses, pHYBi ensures that its processes are not only safe and sustainable but also well-rounded in their benefits across all areas of impact.

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